| 质粒名称: | pEG302 22aa SunTag NtDRMcd SUP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115489 |
| 存储实验室: | Steven Jacobsen |
|
载体骨架基本信息 |
|
| 载体名称: | pEG302 |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR，合成生物学 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS,22aa SunTag NtDRMcd SUP |
| 物种来源: | -- |
| 插入大小: | 16867 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pEG302 22aa SunTag NtDRMcd SUP was a gift from Steven Jacobsen (Addgene plasmid # 115489 ; http://n2t.net/addgene:115489 ; RRID:Addgene_115489) |
| 参考文献部分: | Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pEG302 22aa SunTag NtDRMcd SUP 26918 bp ds-DNA circular SYN 16-APR-2019
DEFINITION CRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus
with two guide RNAs.
ACCESSION .
VERSION .
KEYWORDS pEG302 22aa SunTag NtDRMcd SUP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 26918)
AUTHORS Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
TITLE Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9
SunTag systems.
JOURNAL Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
PUBMED 30760722
REFERENCE 2 (bases 1 to 26918)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 16, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..26918
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 4616..4640
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4659..4714
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 4728..8831
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8835..8861
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8880..8900
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8907..8927
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8937..8957
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 9021..9077
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9144..9200
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9267..9323
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9390..9446
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9513..9569
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9636..9692
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9759..9815
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9882..9938
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 10005..10061
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 10128..10184
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
terminator 10283..10990
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(11023..11041)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(11024..11041)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(11065..11082)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(11065..11081)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11074..11096)
/label=M13/pUC Forward
/note="In lacZ gene"
misc_feature 11284..11308
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 12609..13238
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 13667..14740
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 14806..15000
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(15236..15258)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 15344..15484
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 15399..15418
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(15499..15516)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(15670..16258)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(15750..15769)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(17683..18477)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 18902..18926
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 19016..19190
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(19231..20256)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(20323..20999)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind complement(20366..20386)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
misc_RNA complement(21102..21177)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(21659..21734)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(22502..22552)
/codon_start=1
/product="nuclear localization signal from the human T-cell
leukemia virus type 1 (HTLV-1) Rex protein"
/label=Rex NLS
/translation="PKTRRRPRRSQRKRPPT"
CDS complement(22580..22741)
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS complement(22772..22792)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(23912..24622)
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(23952..23972)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 24567..24595
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
CDS complement(24713..24739)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
ORIGIN
1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
541 ttaaaagctc tgttgagcta gactcatatg catccctagg aaacaagttg taatgagttg
601 ctggcctctc ttgggattgt tcatcaaagt agctgtccct acacaccaca acagaatggt
661 gttgtggaga gaaagcacag acacatcctt gagatggcaa gggcacttaa gtttcagagt
721 ggtgtaccta ccaggttttg gggagactgt gtcaagactg ttgtatacct gatcaataga
781 ctccctactc caatcttaca aggcaaatgt ccatatgaac tactctatca gaaacatgcc
841 aagcttgatc acttgagagt gtttgggtgt cttgcttttg cagcaacact gcccaaaggt
901 gacaaacttg cacctagagc caagcgaacc atcttcattg gttattctga gactcaaaag
961 ggttacaggt tgtatgactt ggataataag gtgatcatag tgagcaggga tgtagttttc
1021 agagagtttc agttcccttt caaagagtcc atctcccatg aacctgatat gttcactcga
1081 gcaggttcac ttagctctga agatacagct ttgcagctag tgtataatga tatttttcct
1141 tccagctttc atactgatga tgatgatact ccagttctta ttcctgacat tgtagaggcc
1201 aatgaggata tcactttggg gactcattct acccaaaatc atatcacttc agctgatgct
1261 gcaccttcag gccacctgca tgctcctgca gagtcagttg agtcagctga gcctgaccta
1321 gcaaatgctg aatttgagca ccatactgct gtagctgatc cctctctagt tccagcaaac
1381 ctgcacacta gacctaaacg caatgcaggt cctcccatct ggctcaagga ctttgtgaca
1441 ctgaacaaag gctctaggga tgttccatac cctatagcca actatgtttc ctatgatcac
1501 ttgcctgttc actatcaagc ttatttgagt gctttctcca cttatactga acctaaatcc
1561 ttcaaagaag cagctcaaga tgagaaatgg atggaggcca tgtcccttga gatacaggct
1621 cttgaggata ataacacctg ggagattgtc cctttacccc ctggtaaaca gcctataggg
1681 tccaaatggg tgtacaaaat taaatacaaa gctaatggtg aagttgacag gtttaaggca
1741 aggctagtgg ccaagggata cactcagcaa gaaggccttg actaccatga aactttttct
1801 ccagtggcca aaatggtcac tgtaaaagct gtcatatctg ttgctgcttc caagggctgg
1861 ttccttttcc agatggatgt caacaatgct tttctacaag gtgacctcat ggaagaagtt
1921 tatatgtctc tgcctcaggg ttttcacagc caaggggaga ccaaagtgtg caggctgttg
1981 aatccctcta tggtttaaag caagcatcaa ggcagtggaa catcaagctt accactgtcc
2041 ttatgcaggc tggttttatg caaagtgctt atgatcactc cttgtttacc aaaagagagg
2101 ggactgacct tgtcataatc ctgatttatg tggatgatct gttaataaca ggcagcagca
2161 atgttttgat ttcagaagca aaggcaaccc tgcatcagca tttcaagatg aaagatttgg
2221 gagaactaaa atacttctta ggcattgagg tgctgagatc agaaaaggga atcctactga
2281 accagaggaa gtatgcactg gaactgatat caggtgttgg tctgggaggc tgcagaccag
2341 tgtcaacacc aatggaacag aaccaaaggc tgactactgt agaatatgac aagcacttag
2401 gaaagactga tgatgcagag ttagaggatg tgggttccta tcagagactg gtgggaaagc
2461 ttctctactt gacaatcaca aggccagata tcagctttgc agtgcaggtg ctgagtcaat
2521 tcatgcagca acccaaacag tcacacctgg aagcagcatt gagggttgtg aagtacataa
2581 aaggttctcc aggtgttagg aacgcggccg catgcatatg agtctagctc aacagagctt
2641 ttaacccaaa ttggtacaat agaatacaac tttagatcat aattctcaaa agaaagagat
2701 tccttagcta ttctatctgc cactccattt ccttctcggc ttgtatgcac aagcataaaa
2761 tcctcaaact tgctaagtag atactttatg tcttggataa ttggattgag acttgacaag
2821 cataactttc atgtaaccaa agacacaagt tgctgagaat ccacctcaaa aatgatcttc
2881 ctataattga atcgggataa tgacagcaca gcccatctaa gagcctccac ttctacttcc
2941 agcacgcttc ttacttttac cacagctctt gcacctaacc ataacacctt ccctgtatga
3001 tcgcgaagca cccaccctaa gccacatttt aatccttctg ttggccatgc cccatcaaag
3061 ttgcacttaa cccaagattg tggtggagct tcccatgttt ctcgtctgtc ccgacggtgt
3121 tgtggttggt gctttcctta cattctgagc ctctttcctt ctaatccact catctgcatc
3181 ttcttgtgtc cttactaata cctcattggt tccaaattcc ctccctttaa gcaccagctc
3241 gtttctgttc ttccacagcc tcccaagtat ccaagggact aaagcctcca cattcttcag
3301 atcaggatat tcttgtttaa gatgttgaac tctatggagg tttgtatgaa ctgatgatct
3361 aggaccggat aagttccctt cttcatagcg aacttattca aagaatgttt tgtgtatcat
3421 tcttgttaca ttgttattaa tgaaaaaata ttattggtca ttggactgaa cacgagtgtt
3481 aaatatggac caggccccaa ataagatcca ttgatatatg aattaaataa caagaataaa
3541 tcgagtcacc aaaccacttg ccttttttaa cgagacttgt tcaccaactt gatacaaaag
3601 tcattatcct atgcaaatca ataatcatac aaaaatatcc aataacacta aaaaattaaa
3661 agaaatggat aatttcacaa tatgttatac gataaagaag ttacttttcc aagaaattca
3721 ctgattttat aagcccactt gcattagata aatggcaaaa aaaaacaaaa aggaaaagaa
3781 ataaagcacg aagaattcta gaaaatacga aatacgcttc aatgcagtgg gacccacggt
3841 tcaattattg ccaattttca gctccaccgt atatttaaaa aataaaacga taatgctaaa
3901 aaaatataaa tcgtaacgat cgttaaatct caacggctgg atcttatgac gaccgttaga
3961 aattgtggtt gtcgacgagt cagtaataaa cggcgtcaaa gtggttgcag ccggcacaca
4021 cgagtcgtgt ttatcaactc aaagcacaaa tacttttcct caacctaaaa ataaggcaat
4081 tagccaaaaa caactttgcg tgtaaacaac gctcaataca cgtgtcattt tattattagc
4141 tattgcttca ccgccttagc tttctcgtga cctagtcgtc ctcgtctttt cttcttcttc
4201 ttctataaaa caatacccaa agagctcttc ttcttcacaa ttcagatttc aatttctcaa
4261 aatcttaaaa actttctctc aattctctct accgtgatca aggtaaattt ctgtgttcct
4321 tattctctca aaatcttcga ttttgttttc gttcgatccc aatttcgtat atgttctttg
4381 gtttagattc tgttaatctt agatcgaaga cgattttctg ggtttgatcg ttagatatca
4441 tcttaattct cgattagggt ttcatagata tcatccgatt tgttcaaata atttgagttt
4501 tgtcgaataa ttactcttcg atttgtgatt tctatctaga tctggtgtta gtttctagtt
4561 tgtgcgatcg aatttgtcga ttaatctgag tttttctgat taacagggat catcaacaag
4621 tttgtacaaa aaagcaggct ctttaaagta tttttacaac aattaccaac aacaacaaac
4681 aacaaacaac attacaatta ctatttacaa ttacaaaaaa agttaacatg gacaagaagt
4741 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
4801 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
4861 agaacctgat cggcgccctg ctgttcgaca gcggagaaac agccgaggcc acccggctga
4921 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
4981 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
5041 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
5101 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
5161 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
5221 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
5281 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
5341 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
5401 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
5461 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
5521 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
5581 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
5641 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
5701 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
5761 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacatcgatg
5821 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
5881 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
5941 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
6001 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
6061 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
6121 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
6181 gcgccagcgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
6241 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtac aacgagctga
6301 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
6361 aaaaagccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
6421 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
6481 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
6541 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
6601 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
6661 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
6721 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
6781 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
6841 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
6901 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
6961 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
7021 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
7081 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
7141 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
7201 accaggaact ggacatcaac cggctgtccg actacgatgt ggacgctatc gtgcctcaga
7261 gctttctgaa ggacgactcc atcgataaca aagtgctgac tcggagcgac aagaaccggg
7321 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcgcc
7381 agctgctgaa tgccaagctg attacccaga ggaagttcga caatctgacc aaggccgaga
7441 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
7501 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
7561 agaacgacaa actgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
7621 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
7681 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
7741 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
7801 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
7861 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
7921 caaacggcga aacaggcgag atcgtgtggg ataagggccg ggactttgcc accgtgcgga
7981 aagtgctgtc tatgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
8041 tcagcaaaga gtctatcctg cccaagagga acagcgacaa gctgatcgcc agaaagaagg
8101 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
8161 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
8221 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
8281 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
8341 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
8401 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
8461 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaaacact
8521 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
8581 ctaatctgga caaggtgctg agcgcctaca acaagcacag agacaagcct atcagagagc
8641 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
8701 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
8761 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
8821 tgggaggcga cgcctatccc tatgacgtgc ccgattatgc cagcctgggc agcggctccc
8881 ccaagaaaaa acgcaaggtg gaagatccta agaaaaagcg gaaagtggaa gatgctccaa
8941 agaagaagag aaaggtcgac ggcattggta gtgggagcaa cggcagcagc ggatccaacg
9001 gtccgactga cgccgcggaa gaagaacttt tgagcaagaa ttatcatctt gagaacgaag
9061 tggctcgtct taagaaaggt tctggcagtg gaggttctgg ctccggatct ggtggttcgg
9121 gctcaggcgg gtccggatca ggcgaagaac tgctttcaaa gaattaccac ctggaaaatg
9181 aggtagctag actgaaaaag gggagcggaa gtgggggctc cgggtcgggc tcagggggct
9241 ccggttcggg aggctcaggg tcgggggagg agttgctgag caaaaattat catttggaga
9301 acgaagtagc acgactaaag aaagggtccg gatcgggtgg ttcaggatct ggatccggag
9361 gatcagggtc cggtgggtcg ggctcaggag aggagttact ctcgaaaaat tatcatctcg
9421 aaaacgaagt ggctcggcta aaaaagggca gtggttctgg aggatctggg tcggggtcag
9481 gcgggtctgg atctggggga tctggatctg gtgaagagct attatctaaa aactaccacc
9541 tcgaaaatga ggtggcacgc ttaaaaaagg gaagtggcag tggtgggtcg ggatctggct
9601 ctggtggctc aggctcggga ggttcaggtt ccggggaaga gctactatcc aagaattatc
9661 atcttgagaa cgaggtagcg cgtttgaaga agggttccgg ctcaggagga tctgggtcag
9721 gatcgggggg ttccgggtca ggcgggtccg ggtcaggcga ggaactgctc tcgaagaact
9781 atcatcttga aaatgaggtc gctcgattaa aaaagggatc gggcagtggt gggtccggct
9841 ccggttccgg aggatcggga tctgggggct cgggatccgg ggaggaacta ctttcaaaga
9901 attaccacct cgaaaacgaa gtagctcgat taaagaaagg ttcagggtcg ggtggctcag
9961 gttcgggatc aggtgggtca ggctccggtg gttcaggttc gggagaagaa ttactgagta
10021 aaaattatca tctggaaaat gaggtagcga gactaaaaaa ggggagtggt tctggcggtt
10081 cgggatctgg ctctgggggc tctgggtcgg gagggtctgg gtctggcgag gaattgctat
10141 cgaaaaatta tcatcttgag aacgaagttg ctaggctcaa aaagggctca ggctcaggcg
10201 ggtccgggtc agggtcggga ggttccggat ccgggggatc aggctcaggg taacccgggg
10261 gcgcgccatg ataactagag tcctgcttta atgagatatg cgagacgcct atgatcgcat
10321 gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt gtagctcaga
10381 tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta tcgtattttt
10441 atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat gtacaatatt
10501 aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga tagagcgcca
10561 caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag cggcagaacc
10621 ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt gccccagggg
10681 ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg aactccggtt
10741 ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc cgctctaccg
10801 aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg acttgctgcc
10861 ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg caggtcaaac
10921 cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac aacatgtcga
10981 ggctcagcag gaccggcatg caagctagct tactagtgat attctatagt gtcacctaaa
11041 tctctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
11101 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
11161 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
11221 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
11281 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
11341 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
11401 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
11461 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
11521 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
11581 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
11641 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
11701 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
11761 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
11821 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
11881 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
11941 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
12001 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
12061 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
12121 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
12181 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
12241 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
12301 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
12361 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
12421 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
12481 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
12541 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
12601 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
12661 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
12721 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
12781 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
12841 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
12901 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
12961 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
13021 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
13081 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
13141 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
13201 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
13261 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
13321 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
13381 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
13441 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
13501 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
13561 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
13621 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
13681 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
13741 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
13801 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
13861 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
13921 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
13981 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
14041 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
14101 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
14161 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
14221 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
14281 gcgtacaaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
14341 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
14401 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
14461 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
14521 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
14581 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
14641 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
14701 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
14761 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
14821 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
14881 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
14941 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
15001 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
15061 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
15121 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
15181 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
15241 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
15301 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
15361 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
15421 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
15481 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
15541 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
15601 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
15661 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
15721 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
15781 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
15841 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
15901 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
15961 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
16021 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
16081 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
16141 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
16201 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
16261 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
16321 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
16381 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
16441 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
16501 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
16561 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
16621 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
16681 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
16741 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
16801 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
16861 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
16921 aaagtgccat ggtataaatc cgttgagaag ctgggttggt actggttaag tcgagtaaga
16981 ggaaaagtac aatatgcaga cctaggagcg gaaaactgga aacctatcag caacttacat
17041 gatatgtcat ctagtcactc aaagacttta ggctataaga ggctgactaa aagcaatcca
17101 atctcatgcc aaattctatt gtataaatct cgctctaaag gccgaaaaaa tcagcgctcg
17161 acacggactc attgtcacca cccgtcacct aaaatctact cagcgtcggc aaaggagcca
17221 tggattctag caactaactt acctgttgaa attcgaacac ccaaacaact tgttaatatc
17281 tattcgaagc gaatgcagat tgaagaaacc ttccgagact tgaaaagtcc tgcctacgga
17341 ctaggcctac gccatagccg aacgagcagc tcagagcgtt ttgatatcat gctgctaatc
17401 gccctgatgc ttcaactaac atgttggctt gcgggcgttc atgctcagaa acaaggttgg
17461 gacaagcact tccaggctaa cacagtcaga aatcgaaacg tactctcaac agttcgctta
17521 ggcatggaag ttttgcggca ttctggctac acaataacaa gggaagactt actcgtggct
17581 gcaaccctac tagctcaaaa tttattcaca catggttacg ctttggggaa attatgaggg
17641 gatctctcag cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
17701 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
17761 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
17821 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
17881 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
17941 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
18001 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
18061 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
18121 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
18181 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
18241 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
18301 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
18361 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
18421 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
18481 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
18541 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
18601 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
18661 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
18721 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
18781 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
18841 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
18901 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
18961 ggacgttttt aatgtactga attaacgccg aattaattcg agctctaatt cgggggatct
19021 ggattttagt actggatttt ggttttagga attagaaatt ttattgatag aagtatttta
19081 caaatacaaa tacatactaa gggtttctta tatgctcaac acatgagcga aaccctatag
19141 gaaccctaat tcccttatct gggaactact cacacattat tatggagaaa ctcgagcttg
19201 tcgatcgaca gatcccggtc ggcatctact ctatttcttt gccctcggac gagtgctggg
19261 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
19321 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
19381 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
19441 gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa gctccggatg
19501 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
19561 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
19621 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
19681 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
19741 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
19801 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
19861 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg cgaccggttg
19921 tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgaacggcg
19981 ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt ccggaatcgg
20041 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
20101 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
20161 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
20221 ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc cggatctgcg
20281 aaagctcgag agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa
20341 atgaaatgaa cttccttata tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc
20401 atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg tggttggaac
20461 gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc
20521 agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc
20581 ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag
20641 gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt
20701 atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttca catcaatcca
20761 cttgctttga agacgtggtt ggaacgtctt ctttttccac gatgctcctc gtgggtgggg
20821 gtccatcttt gggaccactg tcggcagagg catcttgaac gatagccttt cctttatcgc
20881 aatgatggca tttgtaggtg ccaccttcct tttctactgt ccttttgatg aagtgacaga
20941 tagctgggca atggaatccg aggaggtttc ccgatattac cctttgttga aaagtctcaa
21001 tagccctttg gtcttctgag actgtatctt tgatattctt ggagtagacg agagtgtcgt
21061 gctccaccat ggtaccgggt ggtttggttt aaacaaaaaa agcaccgact cggtgccact
21121 ttttcaagtt gataacggac tagccttatt ttaacttgct atttctagct ctaaaaccaa
21181 atcattacta gtactatcaa tcactacttc gactctagct gtatataaac tcagcttcgt
21241 tttcttatct aagcgatgtg ggacttttga agattgtttt caacttaaat gggcctatat
21301 aagaaatact attgttcttt cccatataaa tgggcctgct tctcttcttt cagattccca
21361 ggggcctttt gaagattatc ttcatatctt aagaatgaag atgttttatt caatcaaatt
21421 cttgaaggtt cgatgcctaa tcattctaat cctgggacaa actatgaaac aagatacaaa
21481 aactccgaat ggaaagttaa aaagaagaaa acgaaagcta cggttcaaga aaatgtaagc
21541 tgataaacaa aaaaaaactg tatgaacgaa gaagaagaaa aaaagctaag aagaaatgat
21601 gtattgtgcg gaaggcaagt cgagtttccg ttgttcaacg aaagcttgtt taaaaaaagc
21661 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
21721 tctagctcta aaacctctct catctctata tctccaatca ctacttcgac tctagctgta
21781 tataaactca gcttcgtttt cttatctaag cgatgtggga cttttgaaga ttgttttcaa
21841 cttaaatggg cctatataag aaatactatt gttctttccc atataaatgg gcctgcttct
21901 cttctttcag attcccaggg gccttttgaa gattatcttc atatcttaag aatgaagatg
21961 ttttattcaa tcaaattctt gaaggttcga tgcctaatca ttctaatcct gggacaaact
22021 atgaaacaag atacaaaaac tccgaatgga aagttaaaaa gaagaaaacg aaagctacgg
22081 ttcaagaaaa tgtaagctga taaacaaaaa aaaactgtat gaacgaagaa gaagaaaaaa
22141 agctaagaag aaatgatgta ttgtgcggaa ggcaagtcga gtttccgttg ttcaacgaaa
22201 gctttcctaa acgtaacata gcgtaacata gatgacaccg cgcgcgataa tttatcctag
22261 tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg gactctaatc
22321 ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac atgcttaacg
22381 taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt caatcttaag
22441 aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctcaaact tacggccatg
22501 gcgtaggcgg ccttttccgt tgtgatcttc gcggtctcct ccttgttttt ggactagtac
22561 caccaccgct accaccacct tcggttaccg tgaaggtttt ggtagcgtcg tcgtaggtcc
22621 attcaccgtc aacaccgttg tcgttagcgt actgtttgaa aactttttcc gcggtagcag
22681 cgtcaacagc ttcggtggtg gtttcacctt tcagggtttt accgttcagg ataagcttgt
22741 actcttcggt ccgagatcct cctcccgtac ggacctttct cttcttcttt ggagcccctc
22801 cgccaccgcc atgtttatgt ctggacatta tggacttgac caagtccaat atccgaacat
22861 agtcataaaa tagggaagac tctttgcctt caagcccatc cctgctcact ctgttactgc
22921 ctgcaaggtt attgcacggg cttccaccaa ttaccaaatc aaatcctcca aatgactcta
22981 tcagttgctc caagcggtct ccattcagct gctgcacatc gttaaaatgt ataaggttcc
23041 ctctctgatt agtttgctcc caccagcttc tcacaatatc cctgttgact tcagattttt
23101 ccacagaaac cactgtgttt agtggaatac caagacgata aagagcaact tcaccaccgc
23161 caattccaga gaagagtgat aagacattga taccaccggg aaacaagtct ttcaataccg
23221 acaaatggta tgccacagtg tcaacctgga atgaattacc aagcgacttg tatctatcgg
23281 tcctacttat accacctccc ctagtatggt tctttggaaa tcccagtagc atttcaactt
23341 catcaggctc caaaggagca actttgtttc tcccaaccca caccaaattc cacttccgac
23401 attgatcaag aacaaacttc tgaactctca ttggtggctc accatcaaag tcctccacag
23461 ctttcctaat cctattcgtc aattgtgcac ttccaatggc tgtttgcaaa caatttaact
23521 ttgtccgtgg atcccaagat ggccaccatt tcttcgatag gggaagtgcc tcatgaatgg
23581 tacgtggggc aagtggaaac aaaggaaatc tattttcaac cggcagatta tgaatataac
23641 ccctttttct tgcagcagca caaaaatatt tggagtcgac aaactctggc tcaatatcgt
23701 acaaaaatct agaaattgtg tcccacacac cctttggagc tagagccaca ttttcatagt
23761 agaaaaacgg gggtccaaca gcttgctcgg gaagagttct tcgaaccatt gctggaagtg
23821 gttcggtagg aaccccaaac ccaatcatag gtttgggcaa acgaattgtc tcccctccgc
23881 caccgcccgt acgaccggtc cgacctccac ctttgtagag ctcatccatg ccatgtgtaa
23941 tcccagcagc agttacaaac tcaagaagga ccatgtggtc acgcttttcg ttgggatctt
24001 tcgaaaggac agattgtgtc gacaggtaat ggttgtctgg taaaaggaca gggccatcgc
24061 caattggagt attttgttga taatggtctg ctagttgaac ggaaccatct tcaacgttgt
24121 ggcgaatttt gaagttagct ttgattccat tcttttgttt gtctgccgtg atgtatacat
24181 tgtgtgagtt aaagttgtac tcgagtttgt gtccaagaat gtttccatct tctttaaaat
24241 caataccctt taactcgata cgattaacaa gggtatcacc ttcaaacttg acttcagcac
24301 gcgtcttgta ggtcccgtca tctttgaaag atatagtgcg ttcctgtaca taaccttcgg
24361 gcatggcact cttgaaaaag tcatgccgtt tcatgtgatc cggataacgg gaaaagcatt
24421 gaacaccata ggtcagagta gtgacaagtg ttggccacgg aacaggtagt tttccagtag
24481 tgcaaataaa tttaagggtg agttttccgt ttgtagcatc accttcaccc tctccacgga
24541 cagaaaattt gtgcccatta acatcaccat ctaattcaac aagaattggg acaactccag
24601 tgaaaagttc ttctcctttg ctaccgcttc cacctccacc tggatccaag cttccgccgc
24661 ctcctgaacc accaccgcca ctacctcctc ccccagaacc tccgcctcca ccagcgtaat
24721 ctggaacatc gtatgggtag ctgctcacgg tcaccagggt gccctggccc cagtagtcga
24781 acaggccggt cacgcagtag tacagggcgg tgtcgtcgct gcgcaccttg ctcatctgca
24841 ggtacacggt gttcttgccg ttgtccttgc tgatgatgaa gcggtccttc agggcgctgt
24901 tgtagtcggt gatgccgtcg ccccagatca cgccgatcca ctccaggccg cggccggggg
24961 cctggcgcac ccagttcacg ccgtagtcgg tcaggctgaa gccgctcacg gcgcagctca
25021 gcttcaggct gccgccgggc tgcaccaggc cgccgccgct ctccagcagc ttcacctcgc
25081 tgccgccgcc gctgctgccg ccgccgccgc tgccgccgcc gccgctgccg ccgccgccgc
25141 gcttcagctc caccttggtg ccctggccga acacccagtg gttgctgtac cacagggcgc
25201 agaagtaggt ggcgaagtcc tcgggctgca ggctgctgat ggtcagggtg gccttgtcgc
25261 cgatcaggct gccgctgaag cggctgggca cgccgggggc gcggttgttg gtgccgccga
25321 tcaggccctt gaacagcttg ccgggcttct cctgcaccca gctggcgtag ttgctggtgg
25381 tcacggcgcc ggtgctgctg cggcaggtga tggtcacgcg gtcgcccacg ctggcgctca
25441 ggctgctggg gctctgggtc atcacgatgt cggggcccat cagcgctaaa ccctaggtgt
25501 taatcagaaa aactcagatt aatcgacaaa ttcgatcgca caaactagaa actaacacca
25561 gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa attatttgaa
25621 caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct aacgatcaaa
25681 cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac atatacgaaa
25741 ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac agaaatttac
25801 cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa ttgaaatctg
25861 aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga agaaaagacg
25921 aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa taaaatgaca
25981 cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt atttttaggt
26041 tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc ggctgcaacc
26101 actttgacgc cgtttattac tgactcgtcg acaaccacaa tttctaacgg tcgtcataag
26161 atccagccgt tgagatttaa cgatcgttac gatttatatt tttttagcat tatcgtttta
26221 ttttttaaat atacggtgga gctgaaaatt ggcaataatt gaaccgtggg tcccactgca
26281 ttgaagcgta tttcgtattt tctagaattc ttcgtgcttt atttcttttc ctttttgttt
26341 ttttttgcca tttatctaat gcaagtgggc ttataaaatc agtgaatttc ttggaaaagt
26401 aacttcttta tcgtataaca tattgtgaaa ttatccattt cttttaattt tttagtgtta
26461 ttggatattt ttgtatgatt attgatttgc ataggataat gacttttgta tcaagttggt
26521 gaacaagtct cgttaaaaaa ggcaagtggt ttggtgactc gatttattct tgttatttaa
26581 ttcatatatc aatggatctt atttggggcc tggtccatat ttaacactcg tgttcagtcc
26641 aatgaccaat aatatttttt cattaataac aatgtaacaa gaatgataca caaaacattc
26701 tttgaataag ttcgctatga agaagggaac ttatccggtc ctagatcatc agttcataca
26761 aacctccata gagttcaaca tcttaaacaa gaatatcctg atctgaagaa tgtggaggct
26821 ttagtccctt ggatacttgg gaggctgtgg aagaacagaa acgagctggt gcttaaaggg
26881 agggaatttg gaaccaatga ggtattagta aggacaca
//
